Screening Library Constructs 

Make informed decisions about which constructs are better to progress

The new SUPR-CM from Protein Stable is a truly high throughput stability screening system for antibody engineering which uses 96/384-well microplates throughout the process for convenience of sample handling.  With sensitivity beyond all other microplate readers sample amounts are no longer a barrier to screening large numbers of library constructs.  Chemical melt methods for antibody stability analysis offer the highest quality data, with accurate Cm and thermodynamic parameters essential for understanding of the unfolding process. After rapidly acquiring high-quality data, you can choose which of your constructs are most stable and likely to be able to move down the pipeline for further development and analysis.

When we say user-friendly, we mean it!

Past experience has taught you that user friendly software means it does not have the power to perform well or perhaps that great software may be rather complex and difficult to use.  Now is the time for a new experience.  Flexible, easy to use software which can be set up and running in moments and powerful enough to analyse the most complex data.  Full spectrum reading of the fluorescence signal combined with powerful model fitting and integration, delivers high quality data and a full plate of 384 samples can be read in as little as 2.5 minutes.  Data interpretation takes little more and you are ready to go.

A modular approach

The SUPR-CM is available as a stability screening plate reader only or can also be combined with best-of-the-best liquid handling and lab scale automation to offer the full library screening experience.  By using a modular approach, Protein Stable can offer systems for all space and budget requirements and the chance to upgrade at a later date to gain advances in walk-away time and throughput.  The plate reader can also be integrated to existing screening systems, delivering high throughputs and sensitivity without compromising existing set-ups and training.

Not all proteins are the same

Given the diversity of proteins, there is a large variation in unfolding rates and numbers of transition states, thus requiring a flexible approach to the methodology. The SUPR-CM system allows each module to do its job to the best, meaning that there are no compromises in the process – for example, you will not be restricted to a set incubation period for the chemical melts – select a time that is best suited for your protein to allow the reaction to reach equilibrium. Gaining the best chemical melt data facilitates accurate thermodynamic parameters thus informing decisions based on which to progress.